SHIMURA Kiyohito



Postdoctoral Researcher

Date of Birth


Research Fields, Keywords

capillary electrophoresis, isoelectric focusing, bioaffinity, molecular interaction, immunoassay

Mail Address

E-mail address

Current Affiliation Organization 【 display / non-display

  • Duty ,  School of Medicine (Life Sciences and Social Medicine) ,  Department of Cellular and Integrative Physiology ,  Postdoctoral Researcher

Graduating School 【 display / non-display

  • Graduate School of Hokkaido University ,  Faculty of Pharmaceutical Sciences ,  Biological Chemistry ,  Unfinished ,  JAPAN ,  1979.03

Graduate School 【 display / non-display

  • Hokkaido University ,  Graduate School, Division of Pharmaceutical Sciences ,  Pharmaceutical Biochemistry ,  Doctor's Course ,  Unfinished Course ,  JAPAN ,  1979.03

Degree 【 display / non-display

  • Hokkaido University -  Ph. D. (Pharmaceutical Science) ,  1985.03

Career 【 display / non-display

  • 2010.04


Academic Society Affiliations 【 display / non-display

  • Japanese Electrophoresis Society ,  JAPAN

  • The Division of Electrophoresis, The Japan Society for Analytical Chemistry ,  JAPAN

Field of expertise (Grants-in-aid for Scientific Research classification) 【 display / non-display

  • Bioanalytical Chemistry

  • Capillary Electrophoresis


Research Career 【 display / non-display

  • Development and application of the analytical method of molecular interactions for proteins using capillary electrophoresis

    Project Year: 1993  -  Now 

    The development and application of the analytical method of molecular interactions between protein and protein or protein and ligand using capillary electrophoresis

    capillary electrophoresis, molecular interaction, protein, affinity constant, association constant, dissociation constant, ligand, protein-protein

  • capillary isoelectric focusing

    Project Year: 1991  -  Now 

    Research on the principle and applications of capillary isoelectric focusing and microchip isoelectric focusing for rapid and high-resolution separation of proteins and peptides

    isoelectric focusing, protein, peptide, capillary, micro channel, micro fluidic chip

  • Development and application of affinity probe capillary electrophoresis

    Project Year: 1991  -  Now 

    The development and applications of affinity probe capillary electrophoresis (APCE) that allows rapid quantification of antigens at an amol (1E-18 mol) level with identification of an antigen by isoelectric points as a complex with a fluorescence-labeled antibody fragment

    capillary electrophoresis, capillary isoelectric focusing, antibody, fluorescence detection, laser, immunochemical reaction, isoelectric point, antibody-antigen complex, recombinant antibody

Papers 【 display / non-display

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Books 【 display / non-display

  • Capillary Electromigration Separation Methods

    Colin F. Poole (Part:Capillary Isoelectric Focusing) (Page:167-187)

    Elsevier ,  2018.04 ,  ISBN: 9780128093757

    Kiyohito Shimura ,  Amsterdam


  • Recent development of capillary electrophoresis and microchip electrophoresis

    Shimura, K. (Page:89-176)

    Japanese Electrophoresis Society ,  2008.09

    Shimura, K. (editor)

  • Uh-huh! Q & A's in Protein Research

    Toda, T., Hirano, H., Nakamura, K. (Part:What is "affinophoresis"?) (Page:159-160)

    Yodosya ,  2005.10 ,  ISBN: 9784897064888

    Shimura, K. ,  Tokyo

  • The front edge of analytical methods, from scientific engineering to nano-bio

    Umezawa, Y., Sawada, T., Terabe, S. (Part:Gel electrophoresis) (Page:41-51)

    NTS ,  2004.12 ,  ISBN: 4-86043-067-0

    Shimura, K. ,  Tokyo

  • Principles of Biochemistry (Horton, Moran, Ochs, Rawn, Scrimgeour), Third Edition

    (Part:Chapter 11-14) (Page:251-339)

    Tokyo Kagaku Dojin ,  2003.09 ,  ISBN: 4-8079-0575-9

    Emori, Y., Kawasaki, H., Shimura, K., Soukawa, J. Suzuki, K., Kasai, K., Soukawa, Y. ,  Tokyo

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Academic Awards Received 【 display / non-display

  • International-Activity Promotion Award

    2009.09.19 ,  JAPAN

  • Kodama Award (Japanese Electrophoresis Society)

    1997.10.02 ,  JAPAN

Joint Research activities 【 display / non-display

  • Mod-form pattern analysis by on-line coupling of immunoextraction and capillary isoelectric focusing

    Collaboration in College  

    Project Year:2014.04 - 2017.03 

    Investigator(s): Kiyohito Shimura  ,  Member: Toshihiko Nagai

  • Development of APCE method for the analysis of post-administration isoforms of protein drugs in administered patients

    Collaboration in College  

    Project Year:2013.04 - 2014.03 

    Investigator(s): Shimura, Kiyohito  ,  Member: Nagai, Toshihiko

  • Development of a rapid, precise and easy test for EPO doping

    Commisioned research by the investment  

    Project Year:2011.12 - 2012.07 

    Investigator(s): SHIMURA, Kiyohito  ,  Member: NAGAI, Toshihiko

Presentations 【 display / non-display

  • Capillary isoelectric focusing coupled with immunoaffinity: Two micro platforms for analysis of charge variants produced by post translational modifications

    MSB 2018: The 34th International Symposium on Microscale Separation and Bioanalysis , 


  • Immunoelectrophoresis instrument for new bio-markers

    New Technology Presenting Meetings , 


  • The issues and solutions in the analysis of charge variants of erythropoietin by APCIEF

    The 37th Symposium on Capillary Electrophoresis , 


  • Optimization of isoelectric point-variants analysis of erythropoietin by APCIEF

    The 68th general meeting of Japanese Electrophoresis Society , 


  • Investigation of IEF conditions with ultra violet absorption detection using PDMA-coated capillaries

    The 68th general meeting of Japanese Electrophoresis Society , 


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Charge of on-campus class subject 【 display / non-display

  • Fundamental Natural Sciences (Chemistry)

  • Medical seminar

  • Experimental Approach for Natural Science (Chemistry)

  • General methodology for the investigation in natural science

  • Physical chemistry for biological science

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Academic Activity 【 display / non-display

  • Japanese Electrophoresis Society


  • The Division of Electrophoresis, The Japan Society for Analytical Chemistry

    The member of standing committee