We evaluate the specificity and cross-reactivity of antibodies (e.g., reagents, therapeutic antibodies, and biosimilars) using a specially developed protein microarray. In recent years, the quality of commercially available antibodies has become a significant issue. Thus, we evaluated the specificity and cross-reactivity of commercial antibodies using the protein microarray. As a result, the majority of monoclonal antibodies showed non-specificity and cross-reactivity to antigens. In addition, we evaluated the specificity and cross-reactivity of therapeutic antibodies. We can evaluate various antibodies using the protein microarray.
|Printed protein list※||Excel|
※ The “Controls” sheet is a list of samples containing autoimmune disease-associated antigens in addition to false-positive causing antigens such as recombinant protein tags, various immunoglobulins, and nucleic acids.These samples are used to validate the experiment.